Contributions to Zoology, 86 (3) – 2017Xin Tong; Lu Jiang; Bao-Zhen Hua: A unique mating pattern of Panorpodes kuandianensis (Mecoptera: Panorpodidae)
Material and Methods

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Light microscopy and scanning electron microscopy

Fifty adults (30 males and 20 females) fixed in Carnoy’s solution were dissected under a Nikon SMZ1500 microscope. Photographs were taken with a QImaging Retiga-2000R digital camera attached on the microscope and were stacked with the Syncroscopy Auto-Montage software.

For scanning electron microscopy (SEM), the dissected genital structures were cleaned ultrasonically for 90 s, and then dehydrated in a graded ethanol series, freeze-dried for 3 h, coated with gold in a sputter coater, and examined in a Hitachi S-3400N scanning electron microscope (Hitachi, Tokyo, Japan) at 5 kV.